Journal: Advanced Science

Article Title: Cryoshocked Adipocytes Mediated Dual‐Modal Strategy Combining Photodynamic Therapy and Triptolide Palmitate for Pulmonary Metastatic Melanoma Treatment

doi: 10.1002/advs.202414307

Figure Lengend Snippet: Cellular uptake of Ce6‐pTP‐CsA. a) Representative CLSM images and f) analysis of MFI showing the cellular uptake of BODIPY‐stained Ce6‐pTP‐CsA co‐incubated with A375‐M1 cells for 12 h, 24 h, and 48 h (n = 3). Scale bar: 50 µm. b,c) Western blot analysis of FATP1, FABP4, and CD36 expression in A375‐M1 cells treated with PBS, PA, pTP, and Ce6‐pTP‐CsA (n = 3). d) CLSM and g) MFI analysis of Ce6 uptake in A375‐M1 cells for 3, 12, and 24 h (n = 3). Scale bar: 20 µm (n = 3). e) FCM analysis of Ce6 uptake in A375‐M1 cells for different durations (n = 3). (ns, not significant; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: BODIPY 505/515 and ROS assay kit (DCFH‐DA) were purchased from MedChemExpress (USA).

Techniques: Staining, Incubation, Western Blot, Expressing

Journal: Journal of Advanced Research

Article Title: Triphasic regulation of ZmMs13 encoding an ABCG transporter is sequentially required for callose dissolution, pollen exine and anther cuticle formation in maize

doi: 10.1016/j.jare.2022.09.006

Figure Lengend Snippet: ZmMs13 is regulated by ZmMYB33 and required for anther cuticle formation ( A ) Expression pattern comparison of ZmMs13 and ZmMYB33-1/2 in maize anthers from stages S5 to S13 by qPCR analysis. ( B ) Transactivation assay of ZmMYB33-1 and ZmMYB33-2 using a TDLR system in maize protoplasts. ( C ) Transactivation assay of ZmMs13 promoter activity regulated by ZmMYB33-1 and ZmMYB33-2 using a TDLR in maize protoplasts. ( D ) EMSA analysis of ZmMYB33-1/2 binding to the ZmMs13 promoter. ( E ) SEM observation of anther outer surface of WT and ms13-6060 from stages S9 to S13. ( F ) BODIPY dye staining of WT and ms13-6060 anthers from stages S9 to S13. ( G ) Content determination of cutin and wax (G1) and anther internal lipids (G2) in WT and ms13-6060 anthers at stage 13. ( H ) qPCR analysis of eight cutin and wax synthesis-related genes in WT and ms13-6060 anthers from stages S7 to S13. Data are means ± SD, n = 3 (A, B, C, G, and H). The lowercase letters a to d (B and C) indicate significant differences ( P < 0.01). *, **, and *** indicate the significant levels of P < 0.05, P < 0.01, and P < 0.001 by a two-tailed Student’s t test, respectively.

Article Snippet: BODIPY dye staining of paraffin-embedded sections of ms13-6060 mutant and its WT anthers from stages S9 to S13 was detected by BODIPY 505/515 Kit (A fluorescent probe for lipid droplets, GLPBIO) according to the manufacturer’s instruction, and the fluorescence signals were detected under a laser confocal microscope (TCS-SP8, Leica).

Techniques: Expressing, Comparison, Transactivation Assay, Activity Assay, Binding Assay, Staining, Two Tailed Test

Journal: Beilstein Journal of Nanotechnology

Article Title: Effect of silver nanoparticles on human mesenchymal stem cell differentiation

doi: 10.3762/bjnano.5.214

Figure Lengend Snippet: Influence of Ag-NP/ Ag + ions on the adipogenic differentiation of hMSCs. After 14 d of cell culture (bright-field and fluorescence images), Bodipy 493/503 staining was used to visualize lipid vacuoles in cells cultured under adipogenic conditions. hMSCs incubated in the presence of RPMI/10% FCS served as a negative control (A). hMSCs incubated in the presence of adipogenic-differentiation media served as a positive control (C). Cells were incubated with 10 µg·mL −1 Ag-NP (B) or with 1.0 µg·mL −1 Ag + ions (D) for 24 h and were subsequently incubated with pure adipogenic-differentiation media for further 14 d.

Article Snippet: For Bodipy 493/503 fluorescence quantification microphotographs were taken (Olympus CellP, Olympus, Hamburg, Germany) and digitally processed using Adobe Photoshop ® 7.0.

Techniques: Cell Culture, Fluorescence, Staining, Incubation, Negative Control, Positive Control

Journal: Beilstein Journal of Nanotechnology

Article Title: Effect of silver nanoparticles on human mesenchymal stem cell differentiation

doi: 10.3762/bjnano.5.214

Figure Lengend Snippet: Influence of Ag-NP (black bars) or Ag + ions (grey bars) on the adipogenic differentiation of hMSCs after 14 d of incubation. Quantitative analyses of lipid droplet accumulation were performed by measuring the optical density (520 nm) of extracted oil red-stained lipid droplets (A) or by phase analysis of lipid droplets stained with Bodipy 493/503 (B). The data are expressed as the mean ± SD ( n = 3 independent experiments) given as the percentage of cells cultured under adipogenic conditions in the absence of silver. The asterisks (*) indicate significant differences in comparison to the control (* p < 0.05;** p < 0.005).

Article Snippet: For Bodipy 493/503 fluorescence quantification microphotographs were taken (Olympus CellP, Olympus, Hamburg, Germany) and digitally processed using Adobe Photoshop ® 7.0.

Techniques: Incubation, Staining, Cell Culture